Journal: Gene Expression

Article Title: Polyadenylation precedes splicing in vitro

doi:

Figure Lengend Snippet: Time course of splicing of MXSVL RNA. MXSVL precursor RNA was incubated in in vitro processing reactions for the indicated times, prepared, and displayed on a 5% denaturing acrylamide gel. A. Short time course. Splicing and polyadenylation product bands are identified as on Figure 1B at various times of reaction (minutes). B. Quantitation of intermediate and product bands. The gel in A was scanned in a Betagen 603 gel analyzer, and radioactivity was converted to fmoles product and plotted versus time of reaction. Product species are indicated: A+S−, filled circles; S + A−, open circles; S + A +, triangles. LE2 is lariat-exon 2. Released lariat and exon 1 are off the bottom of the gel. C. Quantitation of the total amount of polyadenylation. The total fmoles of polyadenylated RNA was calculated as the sum of the A + S− and S + A+ RNA (filled circles) and plotted versus time of reaction and with the amount of A + S− RNA (open circles).

Article Snippet: The gel in A was scanned in a Betagen 603 gel analyzer, and radioactivity was converted to fmoles product and plotted versus time of reaction.

Techniques: Incubation, In Vitro, Acrylamide Gel Assay, Quantitation Assay, Radioactivity